A polyclonal antibody directed against syringylpropane epitopes of native lignins.
Identifieur interne : 004129 ( Main/Exploration ); précédent : 004128; suivant : 004130A polyclonal antibody directed against syringylpropane epitopes of native lignins.
Auteurs : Jean-Paul Joseleau [France] ; Oskar Faix ; Ken-Ichi Kuroda ; Katia RuelSource :
- Comptes rendus biologies [ 1631-0691 ]
Descripteurs français
- KwdFr :
- MESH :
- analyse : Lignine.
- composition chimique : Paroi cellulaire, Structures de plante.
- immunologie : Lignine.
- Anticorps monoclonaux, Paroi cellulaire, Propane, Structures de plante, Épitopes.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Lignin.
- chemical , immunology : Lignin.
- chemical : Antibodies, Monoclonal, Epitopes, Propane.
- chemistry : Cell Wall, Plant Structures.
- ultrastructure : Cell Wall, Plant Structures.
Abstract
With a view to visualizing the ultrastructural distribution of syringyl lignins in secondary plant cell walls, a polyclonal antibody raised from a synthetic DHP polymer consisting only of syringyl propane units was prepared. To test the reactivity of the antiserum, a mini-dot-blot immunoassay reducing the amounts of substrates and antiserum was developed. A characteristic attribute of the S-antiserum appears to be its specific recognition of sequences of three or more consecutive syringyl units. On ultra-thin sections of model plants of Arabidopsis thaliana, Populus and tobacco, the antiserum allowed us to demonstrate a higher concentration of syringyl epitopes in fibres than in vessels. Variations in the distribution pattern of these epitopes between the three plants examined suggest that the synthesis of syringyl lignins in angiosperms depends on the species.
DOI: 10.1016/j.crvi.2004.06.003
PubMed: 15587072
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Ichi" sort="Kuroda, Ken Ichi" uniqKey="Kuroda K" first="Ken-Ichi" last="Kuroda">Ken-Ichi Kuroda</name></author><author><name sortKey="Ruel, Katia" sort="Ruel, Katia" uniqKey="Ruel K" first="Katia" last="Ruel">Katia Ruel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004 Sep-Oct</date><idno type="RBID">pubmed:15587072</idno><idno type="pmid">15587072</idno><idno type="doi">10.1016/j.crvi.2004.06.003</idno><idno type="wicri:Area/Main/Corpus">004152</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004152</idno><idno type="wicri:Area/Main/Curation">004152</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004152</idno><idno type="wicri:Area/Main/Exploration">004152</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A polyclonal antibody directed against syringylpropane epitopes of native lignins.</title><author><name sortKey="Joseleau, 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first="Katia" last="Ruel">Katia Ruel</name></author></analytic><series><title level="j">Comptes rendus biologies</title><idno type="ISSN">1631-0691</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Monoclonal (MeSH)</term><term>Cell Wall (chemistry)</term><term>Cell Wall (ultrastructure)</term><term>Epitopes (MeSH)</term><term>Lignin (analysis)</term><term>Lignin (immunology)</term><term>Plant Structures (chemistry)</term><term>Plant Structures (ultrastructure)</term><term>Propane (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps monoclonaux (MeSH)</term><term>Lignine (analyse)</term><term>Lignine (immunologie)</term><term>Paroi cellulaire (composition chimique)</term><term>Paroi cellulaire (ultrastructure)</term><term>Propane (MeSH)</term><term>Structures de plante (composition chimique)</term><term>Structures de plante (ultrastructure)</term><term>Épitopes (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Epitopes</term><term>Propane</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Plant Structures</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Paroi cellulaire</term><term>Structures de plante</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Wall</term><term>Plant Structures</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Paroi cellulaire</term><term>Propane</term><term>Structures de plante</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With a view to visualizing the ultrastructural distribution of syringyl lignins in secondary plant cell walls, a polyclonal antibody raised from a synthetic DHP polymer consisting only of syringyl propane units was prepared. To test the reactivity of the antiserum, a mini-dot-blot immunoassay reducing the amounts of substrates and antiserum was developed. A characteristic attribute of the S-antiserum appears to be its specific recognition of sequences of three or more consecutive syringyl units. On ultra-thin sections of model plants of Arabidopsis thaliana, Populus and tobacco, the antiserum allowed us to demonstrate a higher concentration of syringyl epitopes in fibres than in vessels. Variations in the distribution pattern of these epitopes between the three plants examined suggest that the synthesis of syringyl lignins in angiosperms depends on the species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15587072</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1631-0691</ISSN><JournalIssue CitedMedium="Print"><Volume>327</Volume><Issue>9-10</Issue><PubDate><MedlineDate>2004 Sep-Oct</MedlineDate></PubDate></JournalIssue><Title>Comptes rendus biologies</Title><ISOAbbreviation>C R Biol</ISOAbbreviation></Journal><ArticleTitle>A polyclonal antibody directed against syringylpropane epitopes of native lignins.</ArticleTitle><Pagination><MedlinePgn>809-15</MedlinePgn></Pagination><Abstract><AbstractText>With a view to visualizing the ultrastructural distribution of syringyl lignins in secondary plant cell walls, a polyclonal antibody raised from a synthetic DHP polymer consisting only of syringyl propane units was prepared. To test the reactivity of the antiserum, a mini-dot-blot immunoassay reducing the amounts of substrates and antiserum was developed. A characteristic attribute of the S-antiserum appears to be its specific recognition of sequences of three or more consecutive syringyl units. On ultra-thin sections of model plants of Arabidopsis thaliana, Populus and tobacco, the antiserum allowed us to demonstrate a higher concentration of syringyl epitopes in fibres than in vessels. Variations in the distribution pattern of these epitopes between the three plants examined suggest that the synthesis of syringyl lignins in angiosperms depends on the species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Joseleau</LastName><ForeName>Jean-Paul</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Centre de recherche sur les macromolécules végétales, UPR CNRS 5301, BP53, 38041 Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Faix</LastName><ForeName>Oskar</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Kuroda</LastName><ForeName>Ken-Ichi</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ruel</LastName><ForeName>Katia</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>C R Biol</MedlineTA><NlmUniqueID>101140040</NlmUniqueID><ISSNLinking>1631-0691</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000939">Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C434469">syringyl monolignol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>T75W9911L6</RegistryNumber><NameOfSubstance UI="D011407">Propane</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="Y">Antibodies, Monoclonal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018514" MajorTopicYN="N">Plant Structures</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011407" MajorTopicYN="N">Propane</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>5</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15587072</ArticleId><ArticleId IdType="pii">S1631-0691(04)00179-9</ArticleId><ArticleId IdType="doi">10.1016/j.crvi.2004.06.003</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Grenoble</li></settlement></list><tree><noCountry><name sortKey="Faix, Oskar" sort="Faix, Oskar" uniqKey="Faix O" first="Oskar" last="Faix">Oskar Faix</name><name sortKey="Kuroda, Ken Ichi" sort="Kuroda, Ken Ichi" uniqKey="Kuroda K" first="Ken-Ichi" last="Kuroda">Ken-Ichi Kuroda</name><name sortKey="Ruel, Katia" sort="Ruel, Katia" uniqKey="Ruel K" first="Katia" last="Ruel">Katia Ruel</name></noCountry><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Joseleau, Jean Paul" sort="Joseleau, Jean Paul" uniqKey="Joseleau J" first="Jean-Paul" last="Joseleau">Jean-Paul Joseleau</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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